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Besides direct detection, pre-column derivatization is an effective technique to enhance chromatographic retention and introduce chromophores, thus improving the detection of carnitines

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There are several benefits associated with this type of IV therapy compared to oral supplementation: Enhanced Absorption: When vitamins are taken orally, they must pass through the digestive system, where they may be partially broken down and lose some of their potency

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FIGURE 6 While triolein assays are often used to determine whether a patient may have LPL deficiently leading to HTG, many patients do not carry an LPL variant leading to compromised LPL activity, and LPL activity can appear normal in the cell-free triolein substrate assay described above

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