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GobyMeds having both available through a single platform is a genuine convenience advantage

Further work is needed to clarify the exact mechanisms modulating the different antioxidant responses of the two species during herpesvirus infection, the costs associated with the antioxidant response, and which factors (e.g., species, environment, life-history stage) affect the magnitude of such costs

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

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All plasmid sequences were verified by Macrogen Europe (Amsterdam, the Netherlands)