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Interaction analysis revealed that cysteine formed conventional hydrogen bonds with TYR A:151, TYR B:119, and GLY B:121, suggesting stabilization through polar contacts between the amino acid residues of TNF- and the thiol or amino groups of cysteine

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These abnormalities are associated with reduced oxygen consumption, diminished adenosine triphosphate (ATP) production, and decreased levels of mtDNA and mitochondrial transcription factor A, accompanied by increased respiratory protein expression in hepatic, adipose, and muscular tissues

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Levels 300-400 ng/mL typical therapeutic targets though individual variation