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Quantitative realtime PCR Total RNA was extracted from lung tissue with TRIzol (Life Technologies)

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Mechanistically, IGFBP-2 can activate STAT3 to increase the transcriptional activity of C-X-C motif ligand 1 (CXCL1), thereby increasing the intracellular expression level of CXCL1, which contributes to the survival of lung cancer cells in the gefitinib environment (136)