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doi: 10.1158/1940-6207.CAPR-17-0423 331 RahmanKWAranhaOPSarkarFH

The conformation of TM6-ECL3-TM7 has been correlated with the distinct signalling profiles of GLP-1 and exendin-P5, and along with the conformation of TM1, TM2, and ECL2, are key receptor domains important for GLP-1R cAMP signalling, and biased agonism 3,13,28,29

Expression and Purification of Exendin-4-Fc Chinese hamster ovary cells (passage 1319) were obtained from the China Infrastructure of Cell Line Resources and expanded in CD-CHO complete medium (Invitrogen, Carlsbad, CA, USA) containing 8 mM glutamine