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Cells were washed once in 1.25 M glycine/PBS and lysed for 60 min on ice with homogenization in 1 mL RIPA buffer (50 mM Tris HCl, pH 8.0, 150 mM sodium chloride, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, protease inhibitors (Complete mini, EDTA-free, Roche))

Compared with lifestyle changes or older anti-obesity medications, our study in Obesity showed that GLP-1 receptor agonist regimens are superior for treating weight regain after bariatric surgery

Methylation Genes (MTHFR, MTR, MTRR) MTHFR and other methylation-related gene variants can impair SAMe (S-adenosyl-L-methionine) production