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Coordinate with both your psychiatrist and weight loss provider to stagger initiation if possible, monitor for overlapping nausea or appetite changes, and watch your mood closely during the first 8 weeks

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The cleared lysate (35 ml) supplemented with 700 l of 2 M imidazole (final concentration 40 mM) was mixed with 1.5 ml Ni-NTA beads equilibrated with 10 ml lysis buffer and incubated at 4 C for 2.5 h with gentle rotation