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A 2030 g of total protein were resolved on 412% NuPAGE BisTris gels (Thermofisher) and transferred to nitrocellulose membrane using a semi-dry iBlot2 kit (Thermofisher)

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Tirzepatide (Mounjaro, Zepbound) Tirzepatide is a dualacting medication that targets both GLP1 and glucosedependent insulinotropic polypeptide (GIP) receptors, which may enhance its effects on appetite regulation and metabolism