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Control and p21 KO cells pretreated with Gem also conserved a larger portion of the existing (m+0) intracellular GSH pool after treatment with erastin2 compared with cells pretreated with DMSO, with weaker effects observed in p53 KO cells (Fig 4G)

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High-performance liquid chromatography quantification of GSH/GSSG, 3-NT and 3-CT To evaluate biomarkers of oxidative stress and damage by high-performance liquid chromatography (HPLC), approximately 200 mg of tissue was minced and homogenized in 500 l of ice-cold phosphate-buffered saline

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