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Yeast two-hybrid (Y2H) assay Full-length sequences of ShD14, ShHTL1, ShHTL3, ShHTL4, ShHTL7, and AtHTL were cloned into the pGBKT7 vector as bait (Clontech), and the coding regions of ShMAX2 and AtMAX2 were cloned into the pGADT7 vector as prey (Clontech)

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