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In diabetic nephropathy, podocyte NOX4derived HO drives podocyte injury and slit-diaphragm defects, thereby promoting albuminuria [345], whereas in diabetic retinopathy PKC-driven and VEGF-driven permeability changes are tightly linked to oxidative stress [346]

The resulting mixture was combined with 80 l of 25 M N-acetylcysteine (internal standard), reduced using 20 l of 1.58 M N-ethylmorpholine and 8 l of 25 mM tris-(2-carboxyethy l) phosphine, and labeled with 8 l of 30 mM monobromobimane