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Oocytes were first fixed in 4% paraformaldehyde and permeabilized using Triton X-100, followed by blocking in 1% BSA

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The principal responses to histamine binding to the H 2 R are stimulation of gastric acid secretion, increased sinus rhythm (cardiac rhythm initiated via depolarization from the sinus node), smooth muscle relaxation, inhibition of antibody synthesis, inhibition of T-cell proliferation and inhibition of cytokine production

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