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After freeze-thaw processing, supernatants were collected and stored at -80C until required ( 2.2 Cell viability assay Cell Counting Kit-8 (CCK-8) (Beyotime Biotech, China) was used to measure the corresponding optical density ratio marker cell viability levels in 96-well plates according to the manufacturers instructions

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As shown in Figures 7A, B , TB001 effectively inhibited HK-2 cells migration and EMT process induced by TGF-1, whereas the PKC agonist PMA significantly promoted cell migration

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