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PMID: 23726509

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Real-time polymerase chain reaction (PCR) was performed with Takyon Rox SYBR master mix (Eurogentec, Seraing, Belgium) in a StepOnePlus (Applied Biosystems, Life Technologies, Foster City, CA, USA) using the following primers: IL-6 F 5ACTGGCAGAAAATAAGCTGAATCTTC 3 and R 5TGATCAAGCAAATCGCCTGAT3