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When performed by qualified medical professionals at a licensed clinic using pharmaceutical-grade vitamin formulas, glutathione therapy is a well-established and safe treatment

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Primer sequences used for qPCR assay were listed in Supplementary Table S2

Contraindications and precautions Consider avoiding DSIP or consulting a physician if: Pregnant or breastfeeding (insufficient safety data) Taking naloxone or other opioid antagonists (may block effects) Using medications metabolized by peptidases (potential interactions) History of immune disorders (theoretical immunogenicity concern) Already using multiple sleep medications Drug interactions Because DSIP degrades via amino-peptidases in blood, medications affecting these enzymes (like the ACE inhibitor captopril) could theoretically alter DSIP metabolism