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For GHK-Cu, avoid buffers containing strong chelators (e.g., EDTA) that can disrupt copper complexation.

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Fresh liver and eppididymal fat were removed, weighted, fixed in 4% formaldehyde (Elabscience, China) in a 1.5 ml Eppendorf tube (NEST Biotechnology, China) or snap-frozen in liquid nitrogen and stored at 80C for further research

Understanding GHK-CU shelf life and storage duration prevents using degraded product

Intranasal delivery has been investigated for peptides such as albumin, exendin/GLP-1, GALP, insulin, leptin, and PACAP, with studies indicating that it can achieve rapid brain exposure while minimizing systemic side effects

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