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In these fatty meats carnitine works synergistically alongside creatine, taurine, carnosine, heme iron, zinc, B vitamins, and vitamin K2 to support nearly every major physiological function

For proliferation analysis, expanded HBs were fixed with 4% PFA, incubated in blocking and permeabilizing buffers (0.1% Triton X-100, 5% normal donkey serum), and then incubated with APC-conjugated Ki67 and other indicated antibodies [1]

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Liver tissues were examined using Hematoxylin-eosin staining, Oil Red O staining and Sirius Red staining

Furthermore, the incidence of bone fracture is only recorded as adverse reactions instead of being part of routine data collection, which may result in less than complete information

Giemsa staining of frozen-thawed spermatozoa (repeated in triplicated) was performed for the acrosome integrity assay (36)