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Contamination prevention Every time you puncture the rubber stopper, you introduce a potential contamination pathway
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the Core Research for Evolutional Science and Technology from the JST (to Masayuki Yamamoto and Hozumi Motohashi)
However, these inconsistences may be related to estrous cycle, as only proestrous females show greater cell proliferation than male rats (Tanapat et al., 1999), although this effect has not always been noted (Lagace et al., 2007)