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The nuclei were counterstained using DAPI (Thermo Fisher Scientific), after which the stained sections were imaged with a confocal microscope (LSM880, Zeiss)

DSIP remains an intriguing area of investigation, but decades after its discovery, many fundamental questions remain unanswered

164 However, similar finding has not been seen in studies of MSU crystals induced NETosis, leading to a valuable direction that needed explored

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Given that GS effectors have no appreciable rate of ATP turnover when supplied with canonical substrates, yet all display perfect conservation at the catalytic arginine, we infer that GS-like effectors possess a distinct catalytic activity, which may involve alternative substrate(s)