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Cell pellets were dissolved in PBS and assessed with two fluorescent stains: propidium iodide (Pi) and bis-(1,3-dibutylbarbituric acid) trimethine oxonol [DiBAC4(3)], allowing the observation of cells with loss of membrane integrity and membrane polarity and with distinct nucleic acid content, respectively

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the amount of cysteine governs the speed and quantity of produced GSH

Independent experts caution that the findings should not be extrapolated to men with normal weight or men whose hormone problems have a different cause