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Mass Spectrometry Applications Mass spectrometry enables: N-terminal truncation identification C-terminal truncation analysis Relative impurity quantification Structural confirmation of shortened variants Why Truncation Monitoring Matters These variants can potentially affect: Product potency Stability Pharmacokinetics Regulatory comparability assessments As a result, truncation products must be carefully monitored throughout biosimilar development

Plasma samples revealed increased levels of acetyl and hydroxyl-butyryl species, an elevation of fatty AC intermediates, especially medium-chain unsaturated and dicarboxylic species

Nutrients, 13 (7), 2228

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Below, we summarize studies that remark the importance of the cholinergic immune signaling and circulating choline-containing lipids on lymphocyte differentiation, activation, and function