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2D echocardiography ruled out endocarditis

N = 25 for each treatment group

Its also key to draw the B12 solution right to avoid contamination and get the right dose

doi: 10.1016/j.intimp.2019.02.025 62 SalehiBVendittiASharifi-RadMKrgielDSharifi-RadJDurazzoAet al

Real-Time PCR Total RNA was extracted from cells by acid guanidinium thiocyanate-phenol-chloroform extraction method, and complementary (c)DNA was synthesized using 1 g total RNA in a 20 L RT reaction mix containing 0.5 g/L of random primers, 0.1 mM dNTP, 0.1 M DTT and 5 first strand buffer