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For DDB1 , it was cloned with N-terminal His 6 tag

This was supported by aggravation of WRS-induced damage with the attenuation of tissue release of NO, probably by constitutive NOS (cNOS) inhibitors, resulting in fall in tissue generation of NO and impairment of the gastric microcirculation (35,36)

Chromatography was performed on a Kinetex 2.6 m HILIC 100 , 100 2.1 mm internal diameter column (Phenomenex), with a constant flow rate of 250 l/min and a solvent gradient from 20% Mobile Phase B (50:45:5, ACN:H 2 O:100 mM AmFm in H 2 O) to 22% B at 5 min, 40% B at 10 min, 60% B at 13 min, 80% B at 15 min, and 99% B at 20 min

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