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As biological matrix can metabolize spiked pure compound we added each NAD form individually at different stages of the extraction (Supplementary Table 1) and followed recovery in enzymatic assays

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Collectively, the gut microbiota dysbiosis mediated by these specific bacterial populations may jointly promote the occurrence and progression of OA through multiple pathways such as regulating the hosts immune-inflammatory response, metabolic disorders, and oxidative stress, providing novel targets and strategies based on gut microbiota regulation for the prevention and treatment of OA