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Some cytokines, such as TNF- and TGF-, increase mucin secretion through the interaction of the EGFR and reactive oxygen species pathways as well as generation of small nucleotides which trigger mucin granule exocytosis such as cyclic-di-GMP, ATP, or cAMP (Perez-Vilar et al., 2005
Use a proper reconstitution ratio
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a lower concentration makes them easier to read
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Staying hydrated is critical
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