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Schoell, S

Retention of this unmodified histidine in retatrutide preserves potent glucagon receptor agonism, which is a defining structural distinction compared to tirzepatide, where this position is also histidine but the molecule lacks meaningful glucagon receptor activity due to other sequence differences

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d - g and o - s , Treatment of DIO mice in metabolic cages with once-daily s.c

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Culture medium was comprised of Dulbeccos Modified Eagle Medium (DMEM) containing 25 mM glucose, 10% fetal bovine serum (FBS), 100 units ml 1 penicillin G, and 100 g/ml streptomycin