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Full therapeutic benefit typically becomes evident within three to six months, with many patients experiencing sustained pain relief that persists long after the treatment course ends

2.15 Real-time quantitative PCR Total RNA was extracted from aortic tissue and endothelial cells using TRIzol reagent (Takara Bio Inc., 9108), following the manufacturers instructions

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Nat Rev Drug Discov 10(4):292306 Priyadarsiny P, Khattar SK, Malik R, Udupa V, Seshaiah A, Rahman S, Shingatgeri VM, Bora RS, Saini KS (2008) Differential gene expression analysis of a known hepatotoxin, N -acetyl- p -amino-phenol (APAP) as compared to its non-toxic analog, N -acetyl- m -amino-phenol (AMAP) in mouse liver

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