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Peer review Peer review information Nature Metabolism thanks Timo Mller and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

In brief, trypsinized cells were washed three times with PBS and lysed in lysis buffer (5 mM triethanolamine, 150 mM NaCl, 4% SDS, 2 units benzonase, 5 mM PMSF, pH 7.4) with protease inhibitor cocktail followed by adding EDTA to a final concentration of 5 mM

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Vitamin D's role in perimenopause extends beyond bone: its receptors in pancreatic beta cells, vascular smooth muscle, and brain neurons make deficiency a multisystem liability during the hormonal transition

Based on publicly available disclosures, Sprout Health represents one platform operating in this category that provides detailed information across these factors