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you have simply diluted the concentration

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The same label also advises that the vial should be swirled to mix and not shaken

After viability determination with Trypan Blue (Thermo Fisher Scientific, Countess cell counter) purified CD14+ mononuclear cells were resuspended in complete RPMI Media (RPMI 1640 with 2 mM L-glutamine (Gibco), 5% Serum Replacement (Thermo Fisher Scientific), 5 mM HEPES (Gibco), 1% of 100X MEM nonessential amino acids (Gibco), 1% of 10,000 U/ml Penicillin/Streptomycin (HyClone), 1 mM sodium pyruvate (Gibco), 50 M -mercaptoethanol (Fisher Chemical), and 3.5% of DMEM high glucose (Gibco)) containing 40 ng/ml granulocyte monocyte-colony stimulating factor (human GM-CSF

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