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Whole-cell current clamp recordings were made with a headstage (CV-7B) connected to a computer-controlled amplifier (MultiClamp 700B, Axon Instruments), digitized through a Digidata 1440A Analog/digital converter and acquired at a sampling rate of 10 kHz
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As obesity is considered a chronic disease, a growing sentiment among the obesity medicine community is that obesity medication should be continued long-term rather than for short durations only
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