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Protein samples were separated by 412% SDS-PAGE, transferred onto PVDF membranes (GE Healthcare, Milan, Italy), and incubated with antibodies targeting and Alpha-Smooth Muscle Actin (catalog n A2547, Sigma-Aldrich)

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Replacing l-Ile with d-Ile did not lead to formation of (2 S ,3 S )- t -ES-d-Ile, demonstrating the stereospecificity of Cp1B toward l-amino acids (Supplementary Fig

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