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Gut microbiota inoculum preparation from human feces for ex vivo study One gram of raw fecal sample was mixed with 10 mL of PBS supplement with 0.05% -cysteine in a tube containing glass beads, homogenized the mixture by vortexing for 1 min in an anaerobic chamber (Whitley DG250 Workstation, Don Whitley Scientific Limited, UK), filtered the homogenized fecal liquid through a Falcon 100 m cell strainer, and subsequently used the fecal filtrate for the study

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Iqbal explains how diet and nutrition, physical activity, mindful eating, and other physical and mental health changes all play a part