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After the cells reached ~ 80% confluence under normal culture conditions, the medium was changed to glucose-free DMEM (Gibco) and the dish was placed in an anaerobic chamber (5% CO2, 95% N2) to induce OGD-related cell injury

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Thats where BPC-157 comes in

Psychiatry 55 : 165171 (2004)

They cost significantly more even through Ro's telehealth platform and the cost is compounded by Ro's separate membership fee