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PBMCs were again resuspended in warm RPMI with 5% human AB serum, and 2mL of PBMCs was aliquoted and stored for future assays while the rest was distributed at 6 x 10 5 cells/well onto 24-well plates (Corning, NY, USA) coated with.001% poly-lysine (Sigma, St

First isolated from human plasma in the early 1970s, it has since attracted sustained scientific attention for its role in a broad spectrum of biological processes

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37, 38, 39 Taken together, the findings of this study support our hypothesis and a role for glutathione redox imbalance and oxidative stress in the neuropathology of autism