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Studies of human adipose tissue in vivo

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The release of the nitrobenzoic acid group as nitro-2-thiobenzoate anion (TNB ) creates a yellow anion that can be measured spectrophotometrically at a wavelength of 412 nm

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As new skin cells generated under better antioxidant protection gradually replace older, damaged cells (a cycle that takes roughly 28-40 days), the cumulative effect becomes visible