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Data represent the mean SD of n = 4 experiments performed in biological replicate

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Cell Metab (2019) 30(5):86576.e5

Melatonin normalized the decrease in oxygen consumption rate under oxidative stress in aging HGL5 cells To assess melatonins impact on mitochondrial function in aging HGL5 cells under oxidative stress, we measured OCR using a Seahorse bioenergy analyzer

Dose, timing, and duration get written out for you, not left as a vague "take as needed." The amounts are set per person from your labs and your goal, then adjusted at reassessment

Cell 2015;162:44151