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Computational analysisscRNA data processing and reconstruction of context-specific metabolic models Prior to the reconstruction of single-cell metabolic models, scRNA datasets (Methods: scRNA Datasets) were downloaded from NCBIs Gene Expression Omnibus 89 for pre-processing with Seurat 90 and StanDep 20
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Assessing composite confirmed disability accumulation (composite CDA), which evaluates worsening of EDSS, the 9-hole peg test (9-HPT) and timed 25-foot walk test (T25FWT) for the upper and lower extremity functions, respectively, better capture aspects of disease progression potentially missed with the EDSS, improving the sensitivity in detecting subtle progression [14,15,16,17,18,19]
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