Mechanistically, these data, together with observed decrease in proton leakage, indicated profound changes in mitochondrial function leading to energy depletion
Cells were then washed three times with PBS, counterstained with DAPI, and mounted using Fluoromount-G mounting medium (Invitrogen, 00-4959-52)
In addition, as migraine prophylactic agents, 400 mg/day of riboflavin was comparable with 500 mg/day of sodium valproate and 100 mg/day of riboflavin was comparable with 80 mg/day of propranolol, with no statistical differences between groups in their prophylactic actions, with a more favorable side effect profile in the riboflavin groups (100)
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