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Not for human consumption, therapeutic use, or clinical application

doi:10.1186/s13098-024-01526-2 33

The sections were stained using an Oil Red O staining kit (lot number 20180528, Nanjing Jiancheng Bioengineering), as described previously ( Western Blot Analysis Liver tissue was homogenized in lysis buffer (150 mM NaCl, 1% Nonidet P-40, 0.1% SDS, 50 mM Tris-HCl pH 7.4, 1 mM EDTA, 1 mM PMSF, and 1 Roche complete mini protease inhibitor cocktail)

Diabetic neuropathy is coded using the E11.4x series

20% acetic acid was added followed by 0.6% thiobarbituric acid, and the samples were boiled until pink colour developed

doi: 10.1016/j.jaci.2020.08.017 65 Di DonatoGdAngeloDMBredaLChiarelliF