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Excessive alcohol use activates cytochrome P450 2E1 (CYP2E1), producing harmful reactive oxygen species (ROS) and reducing glutathione, a vital antioxidant
All samples were prepared at the research laboratory of pharmaceutics department, faculty of pharmacy, Minia University
AAV-mediated acute knockdown of Gpr146 confirms its role in promoting hepatic steatosis and adiposity To further validate the regulatory role of GPR146 in hepatic lipid accumulation and to rule out the possibility that the phenotypes observed in germline knockout mice result from developmental compensation, we employed an AAV-mediated approach to acutely knock down Gpr146 in adult mice
However, results differ, and larger studies are still needed
