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The solution should remain consistently light yellow or clear throughout the 28-day use window

MTX-induced liver toxicity is characterized by increased levels of aminotransferases, the development of hepatic steatosis, and progression to liver fibrosis and cirrhosis

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BWFI has lower osmolality and is not isotonic

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Cellular redox homeostasis is jointly maintained by two thiol-dependent antioxidant systems, the GSH and Trx systems, which exhibit redundant functions via inter-system electron transfer capabilities (69)