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With the widespread development and application of drug-loaded nanosystems in tumor therapy, numerous studies have confirmed that various nanoparticles can exert anti-tumor activity by regulating one or more immune cells in the tumor microenvironment (TME), including depleting M2-type TAMs in the TME, limiting the recruitment and localization of TAMs, reprogramming M2-type TAMs into M1-type TAMs, enhancing NK cells homing or function, promoting DCs maturation, inhibiting TANs recruitment or altering their polarity, reducing circulating and tumor-infiltrating MDSCs, altering MDSCs phenotype or inhibiting MDSCs functions (including nanocapsules, metal-organic frameworks, micelles, polymers, dendritic macromolecules, liposomes, and other material nanoparticles), promoting CD8 + T cells proliferation or activity and reducing the number of Tregs (including nanoparticles composed of liposomes, gels, cerium, and selenium, hyaluronic acid-modified nanoparticles, nanocapsules, nanovesicles, other material nanoparticles, and nanoparticles combined with other treatment modalities), besides, some nanoparticles can exert anti-tumor activity by regulating two or more types of immune cells in the TME

After natural cooling, the slides were placed in PBS (pH 7.4) and washed thrice for 5 min each

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These differences may influence chromatographic retention, mass-spectrometric response, surface adsorption, membrane interaction, solubility and recovery from experimental matrices

The CXCL12/CXCR4 axis has been shown to play an important role in PC cell proliferation, migration and invasion [53,54,55]