After their nucleotide sequences were confirmed, the coding regions of AsGGT1 , AsGGT2 , and AsGGT3 were cut out as Kpn I- Xho I fragments and were inserted between the Kpn I and Xho I sites in the yeast expression vector pYES2 (Life Technologies)
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In fed-batch fermentation, the #14 mutant achieved 1.98 g/L of glutathione using molasses and CSL as carbon and nitrogen sources, comparable to yields from expensive complex media containing peptone and yeast extract
[DOI] [PMC free article] [PubMed] [Google Scholar] Rnnberg E, Calounova G, Pejler G (2012) Mast cells express tyrosine hydroxylase and store dopamine in a serglycin-dependent manner
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