Let's be honest, these are easy errors to make if you're not paying close attention, but they are devastating to your research outcomes
With the former one, I had to calculate the amount on a teaspoon
The homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays, which were conducted according to the manufacturers instructions
Salidroside induces mitochondrial dysfunction and ferroptosis to inhibit melanoma progression through reactive oxygen species production
" "For patients diagnosed with diabetes mellitus on a very low energy/liver reduction diet for the purposes of surgery, sodium-glucose cotransporter-2 inhibitors should be stopped at commencement of the diet, and adjust diabetes mellitus treatment as necessary." "Written sick-day rules should be provided to patients at pre-operative assessment and at discharge." 3.5 Comparison with Other Guidelines: 3.5.1 American Heart Association (AHA) Guidelines [4] : "In patients scheduled for NCS, SGLT2i should be discontinued 3 to 4 days* days before surgery to reduce the risk of perioperative metabolic acidosis (COR I/LOE C-LD) *Canagliflozin, dapagliflozin, and empagliflozin should be stopped 3 days and ertugliflozin 4 days before scheduled surgery." 3.5.2 European Society of Cardiology (ESC) Guidelines [5] : "It should be considered to interrupt SGLT-2 inhibitor therapy for at least 3 days before intermediate- and high-risk NCS." (Class IIa/Level C) 3.5.3 ESAIC Guidelines [3] : "R12.8: SGLT2 inhibitors (SGLT2i) drugs should be withheld for 3 to 4 days before elective procedures to reduce the risk of euglycemic diabetic ketoacidosis
Theres not necessarily a right or wrong way to go about it, but there are some strategies that you can do to help find what works best for you
