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The serum-free medium was used to starve and culture for 3 h so that HUVECs grow synchronously and are in the stationary phase

However all agreed that loss in muscle mass and functional strength leading to compromised physical activity are the common factors in defining sarcopenia [6, 7]

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We also solved the crystallographic structures of the C408S, C201S/C408S, and C201S CstB mutants to 2.48 , 2.48 and 3.11 resolution, respectively, by molecular replacement with the wild-type enzyme (see Table S1 for crystallographic statistics)

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Jan 2010;26(1):26-32