Co-culture of HUVECs and THP-1 cells When the HUVEC confluency met approximately 6070%, the conditioned medium collected from THP-1 cell supernatants stimulated by hypoxia intervention and ECM was added into 6-well plates at a ratio of 1:1 and incubated for 24 h to simulate cell co-culture
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Clinical results: Phase 2 monotherapy (Lancet 2021, n=706, 26 weeks): 10.8% weight loss at 4.5 mg/week dose
The recommended dose of GBS01 was therefore determined to be 4 g of burdock fruit extract, as no dose-limiting toxicity were observed at any dose levels tested
In the SURMOUNT trials, nausea affected 12 to 18 percent of participants at lower doses and higher percentages at escalating doses