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Example 3: Purification of fusion protein using Nickel NTA chromatography: Permeate having His6-AV-Ubiquitin-Arg 34 GLP-l (9-37) peptide was loaded on to the affinity chromatography (Ni-NTA) which was equilibrated with urea buffer (8M urea+50mM Tris, pH 8.0)

The transfected Sup-T1 and AA8 cells exhibited ~ 8- and ~ 30-fold increase of GPx activity as compared to control cells transfected with vector alone

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