For the mitochondrial OCR assays, mitochondria were plated on Seahorse Biosciences 96-well culture plates (5 g of protein per well) and centrifuged for 20 min at 2,000 g at 4 C to adhere mitochondria to the base of wells
Mouse C2C12 myoblasts were cultured at 37C in an atmosphere of 5% CO 2 in growth medium (GM) consisting of Dulbecco's modified Eagle's medium (DMEM) supplemented with heat-inactivated 10% fetal bovine serum and antibiotics (penicillin and streptomycin)
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Glutathione further helps the immune system by facilitating the efficacy of the white blood cells
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