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Gas Chromatography Mass Spectrometry Aided Diagnosis of Glutathione Synthetase Deficiency

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Slides were washed in PBS and incubated for 2 h in a mixture of PBS supplemented with 0.2% Triton X-100 and 0.1% bovine serum albumin, followed by incubation overnight with the primary antibodies (listed in Additional file 2: Table S2)