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Based on the amount of GSH in the lysate, we calculated the GSH concentration assuming an averaged HeLa cell volume of 4,000 m 3 (refs 15, 51, 52)

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For transfection with GLUD1, PRMT7, AKT1 and PI3KCA plasmid or siRNA against PRMT7, the cultured HEK 293T cells were transfected using EZ Trans Cell Transfection Reagent (Life iLab) while AGS or MFC cells were transfected using Lipofectamine TM 2000 (Invitrogen), in Opti-MEM following the manufacturers instructions

Of course, chronic conditions or severe injuries may take longer to show noticeable improvements, sometimes up to 6 weeks

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